ANALYSIS OF ~3H CAMP AND ~3H CGMP BY HPLC

WU SHUYUN;MAO QINGNAN;LIN ZHIHAO;LAI YUNXIANG Institute of Atomic Energy, P. O. Box 275, Beijing

doi:10.7538/yzk.1987.21.02.0225

WU SHUYUN;MAO QINGNAN;LIN ZHIHAO;LAI YUNXIANG Institute of Atomic Energy, P. O. Box 275, Beijing. ANALYSIS OF [~3H] CAMP AND [~3H] CGMP BY HPLC[J]. Atomic Energy Science and Technology, 1987, 21(2): 225-225. DOI: 10.7538/yzk.1987.21.02.0225

Citation:

ANALYSIS OF ~3H CAMP AND ~3H CGMP BY HPLC

WU SHUYUN;MAO QINGNAN;LIN ZHIHAO;LAI YUNXIANG Institute of Atomic Energy, P. O. Box 275, Beijing

Graphical Abstract

Graphical Abstract

Abstract

Abstract

Using high pressure reversed phase liquid chromatography, ~3H CAMP and~3H CGMP were determined successfully. The column was HC-ODS-SIL-Xcolumn, and the moving phase was MeOH-20mmol/l KH_2PO_4 (10/90 or 3/97,V/V, pH=5.5). The analytical time was less than 10min. The measuring precisionwas 1.5% (UV) and 0.15% (radioactivity). The determination limit was 10~(-9)gand 10~2Bq with the detection efficiency of 62%.

FullText(HTML)

References (11)

Cited By

Turn off MathJax

Article Contents

ANALYSIS OF ~3H CAMP AND ~3H CGMP BY HPLC

Graphical Abstract

Abstract

Catalog

Export File

Citation

Format

Content