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LIU YUYING China Institute of Atomic Energy, P. O. Box 275, Beijing. PREPARATION OF ~(35)S-LABELLED LCYSTINE BY BIOSYNTHESIS[J]. Atomic Energy Science and Technology, 1989, 23(6): 60-60. DOI: 10.7538/yzk.1989.23.06.0060
Citation: LIU YUYING China Institute of Atomic Energy, P. O. Box 275, Beijing. PREPARATION OF ~(35)S-LABELLED LCYSTINE BY BIOSYNTHESIS[J]. Atomic Energy Science and Technology, 1989, 23(6): 60-60. DOI: 10.7538/yzk.1989.23.06.0060
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PREPARATION OF ~(35)S-LABELLED LCYSTINE BY BIOSYNTHESIS

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  • A modified method for the biosynthesis of L-cyctine-~(35)S from baker's yeast(Saccharomyces Cerevisiae) is described. The yeast was cultivated in a sulphur-depleted medium containing carrier-free Na_2 ~(35)SO_4 under bubbling of air during 48hours, yeast proteins were hydrolysed enzymatically into aminoacids, and L-cystine-~(35)S was isolated by ion exchange chromatography on a column. Radioactive yieldfor L-cystine-~(35)S is 16-19% and the obtained products have specific activities of 15-22 mCi/mmol and radiochemical purity better than 95%.
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