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CHEN Da-ming1,2, XIE Hong3, QI Ben-zhong1, ZHANG Ying-mei3, JIA Bing2,YANG Hong-wei1, LUO Zhi-fu1, ZHANG Jin-rong1, JIN Xiao-hai1, MA Da-long3(1. Department of Isotope, China Institute of Atomic Energy, Beijing 102413, China;2. Medical Isotope Research Center, Peking U. Preparation and in Vitro Evaluation of Human Recombinant Annexin V Using for Apoptosis[J]. Atomic Energy Science and Technology, 2004, 38(S1): 182-182. DOI: 10.7538/yzk.2004.38.S1.0182
Citation: CHEN Da-ming1,2, XIE Hong3, QI Ben-zhong1, ZHANG Ying-mei3, JIA Bing2,YANG Hong-wei1, LUO Zhi-fu1, ZHANG Jin-rong1, JIN Xiao-hai1, MA Da-long3(1. Department of Isotope, China Institute of Atomic Energy, Beijing 102413, China;2. Medical Isotope Research Center, Peking U. Preparation and in Vitro Evaluation of Human Recombinant Annexin V Using for Apoptosis[J]. Atomic Energy Science and Technology, 2004, 38(S1): 182-182. DOI: 10.7538/yzk.2004.38.S1.0182
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  • Human recombinant Annexin V was produced by expression in E coli with high efficiency through genetic engineering. The technique procedure concerned in the temperature and time of vector expression, the basic routine and purification of proteins was established in order to obtain a large quantity of Annexin V. The results of SDS-PAGE analysis and the apoptosis detection of single cell of thymocytes of Balb/c mice (using) FITC-Annexin V caused by dexamethasone with availableness show that the mature Annexin V with high purity and biologic activity is obtained by ion exchange chromatography. The results of cell binding assay show that its KD is 8.53 nmol/mL
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