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XU Wen-ge, LIU Yi-bing, HAN Shi-quan, HU Yi-ding, LUO Zhi-fu (China Institute Atomic Energy, P.O.Box 275-39, Beijing 102413, China). Preparation and Application of SARS-associated Coronavirus Spike Protein Antibodies[J]. Atomic Energy Science and Technology, 2005, 39(2): 101-101. DOI: 10.7538/yzk.2005.39.02.0101
Citation: XU Wen-ge, LIU Yi-bing, HAN Shi-quan, HU Yi-ding, LUO Zhi-fu (China Institute Atomic Energy, P.O.Box 275-39, Beijing 102413, China). Preparation and Application of SARS-associated Coronavirus Spike Protein Antibodies[J]. Atomic Energy Science and Technology, 2005, 39(2): 101-101. DOI: 10.7538/yzk.2005.39.02.0101
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  • Four hybridoma cell lines secreting monoclonal antibody against SARS-associated coronavirus spike protein were obtained and at same time the polyclonal antibodies were also got by immunizing sheep, goats and rabbits with SARS-associated coronavirus spike protein respectively. Immunoradiometric assay(IRMA) and enzyme-linked immunosorbent assay(ELISA) were established using above Abs for detecting SARS-associated coronavirus, and best assay results were observed when McAb CS_4C_(11) was used as labeled Ab and PcAb(sheep) as capture Ab.
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