Establishment of Enzyme-Linked Immunosorbent Assay for Sulfaquinoxaline

An indirect competitive enzyme-linked immunoassay(ELISA) was developed using polyclonal antibody to measure the residues of sulfaquinoxaline(SQ) in food. The range of standard curve is 0.3-100 ng/mL. The sensitivity of the assay is 0.2 ng/mL. The intra-assay and inter-assay CVs are 7.8%-10.0%, and 10.5%-12.0%, respectively. The recovery of SQ in milk，honey，chicken and eggs are 93.1%-107.0%，69.0%-104.0%，40.0%-73.6%，50.0%-84.0%, respectively. The correlation coefficient between measured and expected value is 0.997 after serial dilutions of the samples with high concentration of SQ.