Breeding of High Yield Strain Producing Acid-Stable <EM>α</EM>-Amylase by N<sup>+</sup> Ion Beam Irradiation

CAI Xing-wang; DU Lian-xiang; LU Fu-ping

doi:10.7538/yzk.2011.45.04.0467

期刊创办：钱三强

刊名题字：郭沫若

CAI Xing-wang, DU Lian-xiang, LU Fu-ping. Breeding of High Yield Strain Producing Acid-Stable α-Amylase by N+ Ion Beam Irradiation[J]. Atomic Energy Science and Technology, 2011, 45(4): 467-473. DOI: 10.7538/yzk.2011.45.04.0467

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CAI Xing-wang, DU Lian-xiang, LU Fu-ping. Breeding of High Yield Strain Producing Acid-Stable α-Amylase by N+ Ion Beam Irradiation[J]. Atomic Energy Science and Technology, 2011, 45(4): 467-473. DOI: 10.7538/yzk.2011.45.04.0467

Citation:

CAI Xing-wang, DU Lian-xiang, LU Fu-ping. Breeding of High Yield Strain Producing Acid-Stable α-Amylase by N+ Ion Beam Irradiation[J]. Atomic Energy Science and Technology, 2011, 45(4): 467-473. DOI: 10.7538/yzk.2011.45.04.0467

Breeding of High Yield Strain Producing Acid-Stable α-Amylase by N⁺ Ion Beam Irradiation

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Bacillus subtilis BF7658, which produces medium-temperature α-amylase, was implanted with N⁺ ion beam to breed mutants. Under the optimal fluence of 1×10¹⁶ cm^-2, a mutant TCCC 11525 producing the acid-stable and medium-temperature α-amylase was obtained. The activity of the mutagenised enzyme is 207 U/mL.

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Breeding of High Yield Strain Producing Acid-Stable α-Amylase by N+ Ion Beam Irradiation

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Breeding of High Yield Strain Producing Acid-Stable α-Amylase by N⁺ Ion Beam Irradiation