Damage Effect of γ-rays on <EM>Bacillus Subtilis</EM> Vegetetive Cells

CHEN Xiao-ming; LIU Fang; ZHENG Chun; LI Xiao-yan; ZHANG Jian-guo; YAN Wan-li

doi:10.7538/yzk.2011.45.07.0875

Atomic Energy Science and Technology > 2011 > 45(7): 875-879. > DOI: 10.7538/yzk.2011.45.07.0875

CHEN Xiao-ming, LIU Fang, ZHENG Chun, LI Xiao-yan, ZHANG Jian-guo, YAN Wan-li. Damage Effect of γ-rays on Bacillus Subtilis Vegetetive Cells[J]. Atomic Energy Science and Technology, 2011, 45(7): 875-879. DOI: 10.7538/yzk.2011.45.07.0875

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Damage Effect of γ-rays on Bacillus Subtilis Vegetetive Cells

1.
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
2.
Institute of Nuclear Physics and Chemistry, China Academy of Engineering Physics, Mianyang 621900, China

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Received Date: December 31, 1899
Revised Date: December 31, 1899

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Abstract

Abstract

In order to investigate the damage effects of γ-rays at cell and molecular level, Bacillus subtilis vegetative cells were irradiated by ⁶⁰Co γ-rays at different absorbed doses. The cell survival rate was examined with the standard plate-count method. The intracellular SOD activity was measured by SOD kit through xanthine oxidase method. DNA double-strand breaks were analyzed by pulsed-field gel electrophoresis (PFGE). The cell survival rate decreases when γ-rays dose increases. A clear relation could not be found between intracellular SOD activity and absorbed dose. The DNA release percentage value and break level value increase obviously with γ-rays dose. Cell survival rate is related to DNA double-strand breaks level. It can be concluded that γ-rays have obviously damage effect on Bacillus subtilis vegetative cell, and the damage effect changes with SOD activity and DSB.
- γ radiation,
- SOD activity,
- double-strand break,
- irradiation sterilization

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References (14)

References

[1]	刘树铮. 医学放射生物学[M]. 北京：原子能出版社，2006.
[2]	SETLOW P. Spores of Bacillus subtilis: Their resistance to and killing by radiation, heat and chemicals[J]. Journal of Applied Microbiology, 2006, 101(3): 514-525.
[3]	周莉薇，陈晓明，张建国，等. 枯草芽孢杆菌耐辐射菌株对紫外线的耐受性及其机理初探[J]. 辐射研究与辐射工艺学报，2009，27(3)：182-185. ZHOU Liwei, CHEN Xiaoming, ZHANG Jianguo, et al. The study on ultraviolet tolerance and tolerant mechanism of a Bacillus subtilis radiation-resistant strain[J]. Journal of Radiation Research and Radiation Processing, 2009,27(3): 182-185（in Chinese）.
[4]	RYDERBERG B, LBRICH M, COOPER P K. DNA double-strand breaks induced by high-energy neon and iron ions in human fibroblasts: Ⅰ. Pulsed-field gel electrophoresis method[J]. Radiation Research, 1994, 139: 133-141.
[5]	HOGLUND E, BLOMQUIST E. DNA damage induced by radiation of different linear energy transfer: Initial fragmentation[J]. Int J Radiat Biol, 2000, 76(4): 539-547.
[6]	周光明，李文建，高清祥，等. DNA双链断裂产额的新算法[J]. 原子核物理评论，2003，20(1)：52-54. ZHOU Guangming, LI Wenjian, GAO Qingxiang, et al. A new method for calculating yield of DNA double-strand breaks[J]. Nuclear Physics Review, 2003, 20(1): 52-54（in Chinese）.
[7]	DHARMENDRA K M, SOUMYAKANTI A, CHERUPALLY K K N, et al. DNA protective properties of vanillin against γ-radiation under different conditions: Possible mechanisms[J]. Mutation Research, 2007, 634(1-2): 69-80.
[8]	CEDERVALL B, WONG R, ALBRIGHT N, et al. Methods for the quantification of DNA double-strand breaks determined from the distribution of DNA fragment sizes measured by pulsed-field gel -electrophoresis[J]. Radiation Research, 1995, 143(1): 8-16.
[9]	FURUTA M, SUWA T, KUWABARA Y, et al. Electron-beam sterilization of laboratory animal diets-sterilizing effect of 10 MeV electrons from a linear accelerator[J]. Exp Anim, 2002, 51(4): 327-334.
[10]	陈晓明，谭碧生，张建国，等. 快中子辐照对枯草芽孢杆菌的灭菌效果研究[J]. 辐射研究与辐射工艺学报，2007，25(3)：166-169. CHEN Xiaoming, TAN Bisheng, ZHANG Jianguo, et al. Study on the sterilizing effects of fast neutron radiation on Bacillus subtilis[J]. Journal of Radiation Research and Radiation Processing, 2007, 25(3): 166-169（in Chinese）.
[11]	DAHM D J, DIKOMEY E. Radiosensitivity of human tumour cells is correlated with the induction but not with the repair of DNA double-strand breaks[J]. Int J Radiat Biol, 1994, 66(5): 553-555.
[12]	周光明，李文建，王菊芳，等. γ射线诱导的肝癌细胞DNA双链断裂[J]. 核技术，2000，23(11)：776-779. ZHOU Guangming, LI Wenjian, WANG Jufang, et al. DNA double strand breaks induced by γ-ray[J]. Nuclear Techniques, 2000, 23(11): 776779（in Chinese）.
[13]	LBRICH M, COOPER P K, RYDBERG B. Non-random distribution of DNA double-strand breaks induced by particle irradiation [J]. Int J Radiat Biol, 1996, 70(55): 493-503.
[14]	PRISE K M, PINTO M, NEWMAN H C, et al. Review of studies of ionizing radiation-induced double-strand break clustering[J]. Radiation Research, 2001, 156(5): 572-576.

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