mRNA Expression of Genes PIG3 and Cell Cycle Change of AHH-1 Cell Induced by Proton

AHH-1 cell was irradiated with proton (21 MeV) at Q3D magnetic spectro-meter of the tandem accelerator to study the dose and time-effect of PIG3 gene mRNA expression in AHH-1 cell. Real-time quantitative PCR results show that PIG3 gene mRNA expression increases with doses at the different time after proton irradiation. And the PIG3 gene mRNA expression changes along with time at each dose. The peak of the PIG3 gene mRNA expression appears at 6 h for every dose except the 8 Gy at 12 h. The results of cell cycle show that the percent of G2/M first increases and then decreases with time. These results show that PIG3 gene mRNA expression in the application of biological dosimeter is potential.