应用国产H BsAg-HBsAb固相放免双测定药盒检测HBsAg和抗-HBs
DETERMINATION OF HBsAg AND ANTI-HBs IN SERA USING SOLID PHASE RADIOIMMUNO-DOUBLE ASSAY KIT
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摘要: <正> 自Blumberg(1965)发现乙型肝炎表面抗原(HBsAg)以来,目前世界上约有2亿HBsAg无症状携带者,其中亚洲和西太平洋地区至少有一亿六千万,而我国就占了一亿人左右。这些无症状携带者实际上是乙型肝炎重要的传染源。有些无症状携带者,因肝功能正常,缺乏临床症状,或因医疗部门检测水平的限制,便作为献血员供血,引起受血者输血后乙型肝炎。所以,严格筛选献血员,阻断乙肝病毒的血源播散,这是涉及全世界的Abstract: Sera of 423 voluntary blood donors that was found negative for HBsAg byreverse passive haemagglutination (RPLA) were collected and retested with solidphase radio-immuno-double assay (RIda) for HBsAg and anti-HBs. 15 cases(3.5%) were found positive for HBsAg. 275 (84.6%) of 320 samples were foundpositive for anti-HBs by RIdA, while 221 (68.1%) were positive by PHA. This method shows higher specificity, sensitivity and simplicity. It is valuablefor screening HBsAg and anti-HBs carriers.
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