Abstract: The data quality of trace element determination in human hair by PIXE isfairly improved, when three measures are adopted: 1, The sample is thinhomogeneous and smooth, especially the sample thicrness is controlled about 0.1-0.2 mg/cm; 2, Acomputer program with mutli-function for the analysis ofcomplex X-ray spectra (PAXMF) is used. Tbe PAXMF program not only gives outthe net areas of characteristic peaks but also can calculate the concentration ofelements in the sample, can subtract the interferences contributed by impuritiesin "blank sample" and can provide practical detection limits for the user; 3,System error is corrected by means of determination of trace elements in humanhair standard material. 16 trace elements are analysed in human hair sample by PIXE as anintercomprarision, the values of 7 elements (Ca, Ti, Mn, Fe, Ni, Zn, Pb) are ingood agreement with the recommended values.