利用辐射接枝化学共价法固定酶的研究

STUDY ON IMMOBILZATION ENZYME USING RADIATION GRAFTING AND CONDENSATION COVALENT

摘要: 文章叙述了利用辐射接枝技术,缩合共价法,使葡萄糖氧化酶固定在聚乙烯、氟46膜上。并用红外光谱法证明葡萄糖氧化酶固定在聚乙烯膜上是有效的。实验结果发现,对于氟46膜,共价率、接枝率、膜蛋白含量和膜酶活力间的相互关系,剂量率低,共价率低时,显示出单位膜酶活力较大。当共价率在4.3％时,单位膜酶活力达到最大值,实验也说明了丙烯酸会影响酶的保留活力。对于聚乙烯酶膜,证明红外光谱法表征是一种简便、有效的方法。

Abstract: The paper is described the immobilization of GOD on polyethylene and F_(46)by radiation grafting and condensation covalent.The GOD on polyethylene film is characterized with IR-spectrum. The results show that the enzyme activity on F_(46) film is high when doserate and covalent yield are low. When covalent yield is 4.3% the enzyme relativeactivity achieves the highest for F_(46) film. The experiment also demostrates thatacrylic acid affects the enzyme relative activity and the method of IR-pectrumcharacter is convenlent efficient for GOD on polyethylene film.