Abstract: A preseparation procedure for the determination of ~(241)Am, ~(148)Nd, ~(149)Sm inan irradiated nuclear fuel is decribed. The distribution coefficients of Am and Ndon the Levextrel are determined. Am, R.E.(Ⅲ) in 5-8 mol/l HNO_3 solutions withNaBrO_3 as oxidant, pass through the first column and are separated from U matrix,trace of Pu, Np, Ce(Ⅳ) and other elements. The solution containing Am, R.E.(Ⅲ)is adjusted to 0.1 mol/l HNO_3 and injected into the second column. Amand R. E. are isolated from Sr, Cs and other fission products and are separatedfrom each other with 0.07 mol/l DTPA-1 mol/l latic acid. The columns arepacked with HDEHP Levextrel. Separation between Am and R. E. (Ⅲ) is satisfactory. Both ~(241)Am and ~(147)Ndare recovered with >99% yield.