Abstract: The concentrations of trace elements in water, grain and soil from Tumotezuoqi of Inner Mongolia are determined by neutron activation analysis(NAA). The analytical quality is verified by the analysis of some standard reference materials. The result shows that the arsenic concentrations in drinking water of endemic arsenism disease area are much higher than those of the allowance limit (<50 μg/L). Fe contents are higher than those of the allowance limit (<300 μg/L), and RE concentrations are about ten times than the elemental composition of freshwater. As and RE contents in wheat and corn of endemic arsenism disease area are higher than those of Beijing wheat. Trace elements in soil of Tumotezuoqi are similar to that in medians of elemental composition of soil.