Abstract: The spallation products from spallation reaction of spallation target triggered by high energy protons in accelerator-driven sub-critical system(ADS), were calculated under different material targets and various energy incident protons respectively using SHIELD code. Then the radiological toxicity of spallation products was studied by applying the concept of annual limit of intake(ALI). It reveals that the toxicity of the spallation products is often on high-rate, especially those alpha emitting rare earths (RE) caused by the incident proton under the ADS required energy largely contribute into overall toxicity of spallation targets. These nuclides will exert a radiological hazard on the biotic environment, if do not transmute them in the radiant field.