磺胺喹噁啉酶联免疫分析方法的建立

Establishment of Enzyme-Linked Immunosorbent Assay for Sulfaquinoxaline

摘要: 利用抗磺胺喹噁啉(SQ)多抗建立酶联免疫分析方法，以测定SQ在动物源食品中的残留。方法学鉴定结果表明，灵敏度为0.2 ng/mL，批内、批间变异分别为7.8%～10.0%、10.5%～12.0%；牛奶、蜂蜜、鸡肉、鸡蛋样品的添加回收率分别在93.1%～107.0%、69.0%～104.0%、40.0%～73.6%、50.0%～84.0%之间。牛奶稀释实验表明，测定值与稀释度呈线性相关，相关系数R=0.997。

Abstract: An indirect competitive enzyme-linked immunoassay(ELISA) was developed using polyclonal antibody to measure the residues of sulfaquinoxaline(SQ) in food. The range of standard curve is 0.3-100 ng/mL. The sensitivity of the assay is 0.2 ng/mL. The intra-assay and inter-assay CVs are 7.8%-10.0%, and 10.5%-12.0%, respectively. The recovery of SQ in milk，honey，chicken and eggs are 93.1%-107.0%，69.0%-104.0%，40.0%-73.6%，50.0%-84.0%, respectively. The correlation coefficient between measured and expected value is 0.997 after serial dilutions of the samples with high concentration of SQ.