Abstract: In order to test the feasibility of electron beam induced mutation on Gladiolus and the effects of various doses of electron beam on flower traits， the corms of gladiolus “Beauty Queen” were irradiated by electron beam with different doses. Flower-colour and inflorescence mutants （M1’and M2’）were obtained, respectively at the doses of 40 Gy and 160 Gy. The two mutants and the parent (CK) and those irradiated with same doses (40 Gy and 160 Gy) were studied. The isozymes of POD, CAT, AMY and EST were tested, it shows that isozymes patterns of gladiolus irradiated at the doses of 40 Gy and 160 Gy a similar to CK, but the mutants’ bands were added or absent compared to CK. Based on band types of isozymes, the cluster analysis was carried out and dendrogram generated using SPSS software version 11.5, it indicates that they may be divided into three groups: CK group (CK, gladiolus irradiated at the doses of 40 Gy and 160 Gy), M1’group and M2’group. The bands of protein subunit were analyzed by SDSPAGE, it shows protein expression is obviously inhibited by electron beam irradiation. But three different protein bands were found in the two mutants, and their molecular weights were 96 kDa, 115.4 kDa, 137.2 kDa, respectively. These special proteins may have relation with regulation and control flower-color and inflorescence. The results indicate that electron beam irradiation is an effective way for inducing flower-color and flower-shape mutants.