Chemiluminescence Immunoassay for Prostate-Specific Antigen
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摘要: 应用鲁米诺-过氧化氢发光体系建立定量测定血清前列腺特异性抗原含量的酶促化学发光免疫分析方法。该方法测量范围为1.5～80 μg/L，灵敏度为0.12 μg/L，批内变异＜5%，批间变异＜15%。回收率为83.8%～118.7%，稀释实验测定值与稀释度呈线性相关，相关系数为0.999。本方法与免疫放射分析方法的相关方程y= 1.07x+ 0.68，相关系数r= 0.97。
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关键词:
- 酶促化学发光免疫分析方法 ,
- 前列腺特异性抗原 ,
- 单克隆抗体
Abstract: The chemiluminescence immunoassay (CLIA) for serum total prostate-specific antigen (T-PSA) was developed. The reaction of luminol with hydrogen peroxide was introduced into this chemiluminescence system. The detection limit is established as 0.12 μg/L (n=10, mean of zero standard + 2SD) and the analytical recovery of PSA is 83.8%-118.7%. The intra-assay and inter-assay CVs vary from 4.4%-5.0% and 6.2%-11.7%, respectively. The experimental correlation coefficient of dilution is found to be 0.999. Compared with immunoradiometric assay (IRMA) kits, the correlative equation is y=1.07x+0.68, and correlation coefficient r= 0.97. The standard range for the method is 1.5-80 μg/L, and it presents good linearity. -
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[1] MAATMAN T J. Comparative analysis of fluctuation of serum tumor markers in advanced cancer of prostate[J]. Urology, 2002, 42(6):672-676. [2] BARAK M, MECZ Y, LURIE A, et al. Evaluation of prostate-specific antigen as a marker for adenocarcinoma of the prostate[J]. Lab Clinical Method, 1989, 113: 598-603. [3] HAUKAAS S, SKAARLAND E, HALVORSEN O J, et al. Cushing’s syndrome in prostate cancer: An aggressive course of prostatic malignancy[J]. Urol Int, 1990, 63(2): 126-129. [4] OHNO K, ARAKAWA H, YODA R, et al. Development of novel high-sensitivity chemiluminescence assay for luminol using thiourea derivatives[J]. Luminescence, 1999, 14(6):355-360. [5] WHITEHEAD T P, KRICKA L J, CARTER T J, et al. Analytical luminescence: Its potential in the clinical laboratory[J]. Clinica Chemica Acta, 1979, 25(9):1 531-1 546. [6] LIN S, HAN S Q, LIU Y B, et al. Chemiluminescence immunoassay for chloramphenicol[J]. Anal Bioanal Chem, 2005, 382:1 250-1 255. [7] PORAKISHVILI N, FORDHAM J L, CHARREL M, et al. A low budget luminometer for sensitive chemiluminescent immunoassays[J]. Immunological Methods, 2000, 234:35-42. [8] WEERASEKERA D A, KIM J B, BARNARD G J, et al. Measurement of serum thyroxine by solid-phase chemiluminescence immunoassay[J]. Clinical Biochemistry, 1983, 20:100-104. [9] WANG M C, VALENZUELA L A, MURPHY G P, et al. Purification of a human prostate specific antigen[J]. Milestone in Urology, 1979, 167(3): 1 226-1 230. [10] 郭春祥，郭锡琼. 介绍一种简单、快速的辣根过氧化物酶标记物酶标记抗体的过碘酸钠法[J]. 免疫学杂志, 1983, 33: 97-100.
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