Abstract: Microscopic distribution of ¹⁰B in sub-cellular tissue is significantly important in microdosimetry of boron neutron capture therapy (BNCT) for measuring apoptosis rates of cell and deposited dosage in cell nucleus. A method for measuring concentration of ¹⁰B in sub-cellular tissue using solid state nuclear track detector （CR-39） was studied. Cell position labeled by laser, optical microscopy scanning, electron microscopy scanning and image reconstruction were utilized to obtain the track density of cell organelle. Based on the result of macro-level measured by ICP-AES, concentrations of ¹⁰B in cell organelle were calculated respectively. The result demonstrates that microscopic distribution of ¹⁰B in sub-cellular tissue could be detected by combining solid state nuclear track detector with laser label and electron microscopy scanning.