Abstract: A self-displacement gas chromatography (SDGC) system using two columns packed with Pd was fabricated for deuterium enrichment. The deuterium enrichment performance of the fabricated SDGC system was investigated and discussed. The experimental results show that the maximum deuterium concentration of effluent gas is up to 31.6% from 2.0% of the sample gas, and 67.3% from 5.0% for SDGC system. The structure of the system is simple and it can be applied to deuterium enrichment from hydrogen isotopes of low deuterium concentration.