Abstract: Growth bone tissue engineering is one of the creative medical fields in reconstruction of bone defect. It can provide the surface of the material with condition of rich osteoblast multiplication through bioactive materials such as Eprgdnyr (RGD peptide) and cell factor introduced in frame material. A quantity measure of the degree that the pieces of calf bone activated by the radiation of the ultra-violet couple RGD peptide onto the pieces of calf bone under the function of the EDC was given by tracer technique 125I-Eprgdnyr. The result shows that the amount that RGD peptide couples onto the bone pieces has positive correlation to that of Eprgdnyr and EDC, aimed at providing scientific basis for facial modification of bone reconstruct and support material.