Abstract: 13N(p,γ)14O is one of the key reaction in hot CNO cycle, which is important for studying the energy source of evolution of stars. The angular distribution of 13N(d,n)14O at Ecm= 8.9 MeV was measured using 13N beam produced by secondary beam facility at HI-13 tandem accelerator. The ANC of 14O was then extracted to be (29.4±5.3) fm-1. In addition, we derived the asymptotic normalization coefficient (ANC) of 14O by analyzing the 13C(d,p)14C angular distribution based on charge symmetry of mirror nuclei, which agrees with the experimental result. The astrophysical S factor and reaction rate of 13N(p,γ)14O were then calculated using R-matrix theory and were inputted into astrophysical reaction network. It shows the energy generated by CNO cycle in novae using the present data is 5% more than that using the previous ones, which could affect the evolution of novae.