Abstract: The treatment of uraniumbearing wastewater from uranium mine and using microorganism to treat wastewater were paid much attention to environmental researchers. Based on column experiments, we investigated the potential using sulfate reducing bacteria（SRB） and zerovalent iron（ZVI） to synergetic treat contamination in wastewater such as sulfate, uranium, etc. SRB+ZVI can effectively remove contamination U(Ⅵ) and SO2-4 in wastewater. The removal rate is 99.4% and 86.2% for U(Ⅵ) and SO2-4, respectively. The pH of wastewater can be basified to neutral. U(Ⅵ) and SO2-4 as electron acceptor of sulfate reducing bacteria are removed by biological reduction. The corrosion of ZVI is benefit to enhance the pH of wastewater, forms anaerobic reducing environment, strengthens survival and metabolism reaction of SRB, and plays a synergetic enhancement.