生防菌BJ1离子辐照突变高效菌株筛选及对黄瓜枯萎病的生防效果

Screening of Mutation High-Yielding Biocontrol Bacterium BJ1 by Ion Beam Irradiation and Effect of Controlling Fusarium oxysporum cucunerinum Disease

摘要: 枯草芽孢杆菌BJ1是一种在真菌病害防治中发挥重要作用的生防因子，为进一步提高它的抑菌能力，获得生防效果更好的高效菌种，利用不同能量和剂量的¹²C⁶⁺对生防菌BJ1进行了离子辐照处理。研究结果表明：离子辐照生防菌BJ1的最适宜剂量为200～400 Gy，传能线密度（LET）为60 keV/μm；突变菌株的抑菌能力比BJ1提高了2%～21%；不仅防病效果比BJ1提高了17.48%，而且对植物具有更好的促生长作用。

Abstract: BJ1 of Bacillus subtilis is an important biocontrol factor in control of fungus disease. In order to improve the antagonistic ability of the strain, and obtain high-efficiency strains,¹²C⁶⁺ of different doses and linear energy transfer (LET) was used to irradiate the biocontrol bacterium BJ1. The optimum dose and LET of ion beam irradiation for the BJ1 are 200-400 Gy and 60 keV/μm, respectively. The antagonistic ability is increased by 2%-21%. The control effect of mutation to Fusarium oxysporum f. sp. cucunerinum is increased by 17.48% over that of BJ1, and mutation also has better plant growth promoting effect.