Abstract: To identify the related proteins about radiosensitivity (RS) of colorectal cancers, as well as make the personal therapeutics for patients, the specimen of transplanted tumor raised by the colorectal cancer cells, LOVO and SW480 with different radiosensityvity, were examined by the fluorescence differential in-gel electrophoresis (DIGE) after labeled with CyDye DIGE fluors Cy3, Cy5 and Cy2. As the result, intensity changes of 35 spots were detected with statistical significance. 18 protein spots of them are up-regulated in tumor specimen of LOVO, otherwise 17 are up-regulated in specimen of SW480. And 27 of them were identified by MALDI-TOF-MS successfully including 17 in LOVO and 15 in SW480. These differential expressed proteins may become the mocular markers and have a key role in predicting the RS of colorectal cancer.