Abstract: Triangular silver nanoplates on the surface of glass substrate were synthesized by small triangular silver nanoplate seeds with the assistance of trisodium citrate dihydrare. The substrates were characterized by transmission electron microscopy, scanning electron microscopy, X-ray diffraction, absorption and fluorescence spectroscopy, respectively. The results show that the triangular silver nanoplate of the substrates finally grows up to 300 nm with the volume increasing of growth solution, and some of nanoparticles stack forms bilayer. A new broad band appears in the absorption spectra of the substrates due to the interparticle dipole-dipole coupling of surface plasmon resonance response of the triangular silver nanoplate particles, which red shifts in 600-800 nm as the particles grow up, indicating the intensification of the coupling. The substrates have an emission band centered at 400 nm in their fluorescence spectra under excitation at 215 nm, the fluorescence intensity shrunk as the average size of the triangular silver nanoplate particles increases.