Abstract: The Ru/γ-Al2O3 catalyst was prepared via incipient impregnation method, and the catalytic properties in the hydrogen-deuterium exchange of methane have been examined over γ-Al2O3 supported Ru catalyst. The effects of various parameters, such as the temperature of reaction, the flow of reactant, the ratio of HD/CH4, on structure and performance were investigated and catalysts were measured through the H2-TPR, XRD. The results indicate that the Ru atoms are in the support interior of the Ru/γ-Al2O3 catalyst and the stronger interaction between Ru and support on the Ru/γ-Al2O3 catalyst. The Ru/γ-Al2O3 catalyst possesses higher catalytic activity and stability in the hydrogen-deuterium exchange of methane.