Abstract: The determination of uranium isotope abundance is the important analysis item in nuclear fuel cycle. Based on neutron activation analysis, a new method was presented for determination of uranium isotope abundance by fission induced by fast neutron generated by T(d, n)⁴He. The exponential relation between different abundances of ²³⁵U and the averaged fission yields ratio of special fission production was used in this method. Through a series of experiments, the working curve selected the relationships between abundances and the ratios of fission yields (〖AKY-〗(⁹²Sr)/〖AKY-〗(¹⁰⁵Ru), 〖AKY-〗(⁹²Sr)/〖AKY-〗(¹³⁵I)). The simulation samples were measured. The relative deviations are 0.8% and 1.8% respectively, RSD is excelled 3%, and the result is coincident with that of γ spectrometry method.