γ辐照对枯草芽孢杆菌营养体的损伤

陈晓明; 柳芳; 郑春; 李晓燕; 张建国; 严万里

doi:10.7538/yzk.2011.45.07.0875

γ辐照对枯草芽孢杆菌营养体的损伤

1.
西南科技大学生命科学与工程学院,四川绵阳621010
2.
中国工程物理研究院核物理与化学研究所,四川绵阳621900

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出版历程
- 收稿日期: 1899-12-31
- 修回日期: 1899-12-31
- 刊出日期: 2011-07-19

Damage Effect of γ-rays on Bacillus Subtilis Vegetetive Cells

1.
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
2.
Institute of Nuclear Physics and Chemistry, China Academy of Engineering Physics, Mianyang 621900, China

摘要

摘要: 选用不同剂量γ射线辐照枯草芽孢杆菌营养体，分别用细胞计数、黄嘌呤氧化及脉冲场凝胶电泳法分析了辐照后的细胞存活率、胞内SOD活性及细胞DNA双链断裂水平。研究发现，随着γ辐照吸收剂量的增大，细胞存活率不断下降；SOD活性随剂量的变化无明显的规律；DNA双链断裂水平与细胞存活率密切相关，DNA的释放百分比和断裂水平值随辐照剂量增加而不断增大。结果表明：γ辐照对枯草芽孢杆菌营养体有较高的灭活能力，其损伤效果可能与SOD活性及双链断裂相关。
- γ辐照 ,
- SOD活性 ,
- 双链断裂 ,
- 辐照灭菌
Abstract: In order to investigate the damage effects of γ-rays at cell and molecular level, Bacillus subtilis vegetative cells were irradiated by ⁶⁰Co γ-rays at different absorbed doses. The cell survival rate was examined with the standard plate-count method. The intracellular SOD activity was measured by SOD kit through xanthine oxidase method. DNA double-strand breaks were analyzed by pulsed-field gel electrophoresis (PFGE). The cell survival rate decreases when γ-rays dose increases. A clear relation could not be found between intracellular SOD activity and absorbed dose. The DNA release percentage value and break level value increase obviously with γ-rays dose. Cell survival rate is related to DNA double-strand breaks level. It can be concluded that γ-rays have obviously damage effect on Bacillus subtilis vegetative cell, and the damage effect changes with SOD activity and DSB.
- γ radiation ,
- SOD activity ,
- double-strand break ,
- irradiation sterilization

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参考文献(14)

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γ辐照对枯草芽孢杆菌营养体的损伤

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出版历程

Damage Effect of γ-rays on Bacillus Subtilis Vegetetive Cells

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出版历程

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