Abstract: The experimental study of prompt neutron decay constant α for 300# pool reactor under mixed core was carried out through a suit of reactor power spectral density measurement system. The two channel continuous current signals of neutron in the reactor were acquired by ionization chamber DL129 which was symmetrically putted in reactor core. The power spectral density, for two channel signals, was computed using the application program of data acquirement and data process analysis. Finally, by using the non-linear least squares method, the prompt neutron decay constant α was fitted. By comparison, the experimental results well accord to the theory calculation within the error range. The deviation can meet the actual need of project.