Abstract: The distributed timing and synchronization system (DTSS) plays an important role in the experiment of EAST, which is one of the national key fusion research projects in China. The purpose of this system is to synchronize each subsystem in EAST experiments. The DTSS based on PXI industry devices and virtual instrument technique, provides reference clock signals with frequency from 1 Hz to 80 MHz. It also produces trigger signals in pre-defined delay from 1 ms to 6 872 s with 10 ns accuracy. This system was able to acquire signals and send output or command to other systems based on signals.