Abstract: Service water system is one of the important safety systems in nuclear power plant, whose failure probability is always gained by system reliability analysis. The probability of equipment failure under the earthquake is the function of the peak acceleration of earthquake motion, while the occurrence of earthquake is of randomicity, thus the traditional fault tree method in current probability safety assessment is not powerful enough to deal with such case of conditional probability problem. An analysis frame was put forward for system reliability evaluation in seismic condition in this paper, in which Monte Carlo simulation was used to deal with conditional probability problem. Annual failure probability of service water system was calculated, and failure probability of 1.46×10^-4 per year was obtained. The analysis result is in accordance with the data which indicate equipment seismic resistance capability, and the rationality of the model is validated.