Abstract: The time response speed of measuring amplifier directly influences the research on ionization chamber’s time response speed. The reasons why the measuring circuit was designed with charge integration amplifier were presented. The mechanism of emerging stepped current signal of ionization chamber unit irradiated by a stable radiation source was analyzed. The method of studying amplifier time response by measuring the changing process of current signal was proposed. The relationship of the amplifier’s time response speed with the detector unit interelectrode capacitance, the amplifier’s input protecting resistance and its feedback integral capacitance was studied through experiment. It is concluded that the amplifier’s time response speed is concerned with the constant time of ionization chamber unit interelectrode capacitance and protecting resistance. And it is not concerned with the feedback integral capacitance. It provides theoretical guidance on designing ionization chamber and measuring circuit.