Abstract: ⁸⁹Rb is an important fission product used for monitoring possible release of fission products from fuel element. The half-life is one of important nuclear parameters. The half-life of ⁸⁹Rb was determined using reference source method with two sets of HPGe detectors by place-relay way. In reference source method, the ratio of net full energy peak areas from the measure nuclide and the reference source was used to avoid the count correction caused by dead time and pileup. For the very short half-life of ⁸⁹Rb, the half-life iterative method was used in data analysis and the translation method was used in data unification. Finally, the measured half-life of ⁸⁹Rb is （14.41±0.04） min.