Abstract: Reference radiation is necessary for calibration of radiation dose monitoring meters, and 60 Co single source radiation facility is an important equipment to produce reference radiation field. Monte Carlo method was used to calculate the uniformity and scattered radiation of the reference radiation field which was produced by 60 Co single source radiation facility, and the design of scattered chamber, collimation and shutter was accomplished. PTW ionization chamber was used to measure the radiation field, and the results show that the scattered radiation and uniformity of the radiation field are in good agreement with the simulation results. The radiation performances meet the design requirements.