Abstract: 14 MeV neutrons originated in acoustic cavitation fusion were determined using copper threshold detector. According to the nuclear reaction of 14 MeV neutrons with copper, the characteristic γ peaks of some radioactive nuclides were measured and the activation parameters of Cu were optimized. With neutron irradiation time of 50 min, 511 keV characteristic γ peak counts of activated copper pieces with or without ultrasonic field after 30 min and 198 min were respectively determined by NaI detector. Measurement results show that the characteristic γ peak counts of ⁶²Cu and ⁶⁴Cu can be respectively determined after 30 min and 198 min. 511 keV characteristic γ peak net count increments are positive values and statistical significance, which indicates that 14 MeV and 2.45 MeV neutron generation rates originated in fusion with ultrasound are greater than that without ultrasound in nuclear reaction liquid. These results verify the nuclear effect of acoustic cavitation (NEAC). The mechanism of NEAC nucleated by neutrons was proposed initially.