Abstract: The ecological transfer parameter is one of the key parameters in environmental impact assessment for nuclear facilities, and it is affected by the biology, water quality and other environmental factors. The parameters vary in several orders of magnitudes in different environments. Pengze site in Jiangxi province and Haiyang site in Shandong province were selected as the cases. Samples of water, sediment, black carp, grass carp, chub and bighead were collected in Pengze site, and samples of water, sediment, pectinid, sea lettuce, stichopus japonicas and sole were collected in Haiyang site. The neutron activation analysis was used to measure the concentrations of element Cs, Ba and Co, and the distribution coefficients and concentration factors were calculated and compared with other published data. Since the two parameters vary in large scope with the region, the site-specific data should be adopted in the environmental impact assessment. In the condition that the concentrations of radioactive isotopes are lower than the minimum detectable limits, the neutron activation analysis could be used to gain the ecological transfer parameters in the specific site for the assessment.