Abstract: The project developing a plastic scintillator detector, undertaken by the Institute of Modern Physics, Chinese Academy of Sciences, is targeted for detection of space particles, reconstructing tracks of incident electron, distinguishing electrons and photons, and identifying heavy ions. In order to construct a test system for the detector, a complete set of data acquisition circuit (DAQ) and upper computer software for test control and date acquisition was designed. The DAQ can receive the data from four front-end electronics (FEE) boards, and complete the data readout of 360 electronics channels on the FEE boards. The DAQ also received the control commands from the upper computer and distributed them to FEEs, received the hit information from the detector and generated trigger signals, and collected the telemetric data of FEEs and sent them to the upper computer for processing after packing. The real-time communication between DAQ and upper computer was achieved via USB bus and RS232 bus. The software of upper computer was developed based on the LabWindows/CVI framework, which can implement the control of FEEs, read and storage of the data from FEEs, and the display of the operation state parameters and other information of FEEs on real time. The system was developed with a compact circuit structure and multiple functions, and the upper computer software has a good human-computer interaction interface. After operation and test practically, the performances of DAQ and upper computer software meet the design requirements, and the system has been applied successfully in the plastic scintillator detector and read-out electronics currently.