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基于64Cu和超顺磁性氧化铁纳米粒子的PET/MRI双模态淋巴显像探针的研究

Investigation of PET/MRI Dual-modal Lymph Imaging Probe Based on 64Cu and Superparamagnetic Iron Oxide Nanoparticle

    摘要
  • 摘要: 采用共沉淀法先合成了由葡聚糖包覆的超顺磁氧化铁纳米粒子（IO-Dex），再用64Cu标记二硫代氨基甲酸二磷酸盐(DTCBP)，获得了64Cu(DTCBP)2。然后，利用DTCBP中二磷酸基团与氧化铁牢固结合的特点，将预标记的64Cu(DTCBP)2与IO-Dex结合，制备出一种用于淋巴结显像的PET/MRI双模态影像探针64Cu(DTCBP)2-IO-Dex。经分离纯化，64Cu(DTCBP)2-IO-Dex的放化纯度大于99%。该影像探针在Balb/C小鼠体内的生物分布结果显示：64Cu(DTCBP)2-IO-Dex 1 h时在腘窝淋巴结（PLN）和髂淋巴结（ILN）的吸收较高，分别为2.31%ID和1.84%ID；肝脏摄取最高，达15.41%ID/g；脾脏在3 h前无摄取。在PET/CT和MRI模式下，均能清晰地观察到腘窝淋巴结的摄取。

     

    Abstract: The dextran-coated superparamagnetic iron oxide nanoparticles were synthesized by the method of chemical co-precipitation firstly, then dithiocarbamate bisphosphonate (DTCBP) was labeled with 64Cu effectively, and 64Cu(DTCBP)2 was obtained. By the binding properties of DTCBP to the iron oxide, a PET/MRI dual-modal lymph imaging probe of 64Cu(DTCBP)2-IO-Dex was prepared by mixing IO-Dex and the pre-labeling 64Cu(DTCBP)2. The radiochemical purity of 64Cu(DTCBP)2-IO-Dex is higher than 99% after purification. The biodistribution of 64Cu(DTCBP)2-IO-Dex in Balb/C mice shows that the uptakes of popliteal lymph node and iliac lymph node are 2.31%ID and 1.84%ID at 1 h after injection, respectively. At the same time, the uptake of the liver is 15.41%ID/g. The radioactivity is no uptake in spleen until 3 h after injection. The uptake of radioactivity in popliteal lymph node can be clearly observed in the mode of PET/CT or MRI.

     

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