Abstract: The improvement was made for the Carbolite biological tritium and carbon burning device and the setting condition of analysis method of environmental biological organic tritium was optimized based on a lot of experiments. After optimization, the water recovery rate of sample can reach more than 80%, the burning of sample is full, and the whole process is much safer. The relative deviation of measurement result for environmental samples by different experimental persons is less than 30%. The result shows the analysis method has a good reproducibility.