Abstract: Experiment and simulation studies for single fuel rod worth were carried out at the light water reactor of Venus-Ⅱ zero-power facility. Five groups of kinetic parameters were calculated by MCNP6 code with five libraries. By plugging the measured double periods into the in-hour equation, five corresponding single fuel rod worths were obtained respectively. Five single fuel rod worths were calculated by slope method using five libraries and the simulation results were compared with the experiment results. The results show that the experiment results of period method exhibit obvious discrepancies due to the differences of kinetic parameters obtained by different libraries. But the simulation results given by the slope method with different libraries are almost consistent. By comparison, a good agreement between the experiment and simulation with JENDL-4.0 is achieved and the relative deviation is less than 1%. Therefore, it is recommended that the kinetic parameters calculated with the JENDL-4.0 should be adopted to process the experiment data and the corresponding single fuel rod worth of period method is (0.237 6±0.015 6) mk. The accurate single fuel rod worth will improve the reliability and accuracy of the follow-up experiments on ADS.