Abstract: Burnup is an important index of nuclear fuel elements after irradiation. And the accurate measurement of burnup value is of great significance for the research of new kind of nuclear fuel element as well as for the optimization of the reload cycle. However, the destructive fuel burnup measurement is a refined chemical analysis in the environment of strong radioactivity. In order to ensure the accuracy of the measured data, establishing a systematic method to control quality is very necessary. In the present paper, the process of quality control method for destructive fuel burnup measurement was introduced from different aspects, such as the suitability of the analytical method, data forecast, mass spectrum interference, sample contamination, multiple method validation and so on. The merits and demerits of the heavy isotope method, ¹⁴⁸Nd, ¹⁴⁵Nd+¹⁴⁶Nd and ¹³⁷Cs monitor methods were analyzed. In addition, the ratios of fission yield were used to estimate the isotopic abundance ratios of Nd and the isotopic abundance ratios of U to estimate the burnup values. Moreover, the detection and elimination means for the mass spectrum interferences caused by Ce and Sm were also analyzed. For the natural background pollution and cross-contamination between samples, the judgment skills and correction methods were studied respectively. Finally, the compare verification methods of burnup value, spike concentration and isotope abundance ratio were also discussed in detail.