Abstract: Fast neutron radiography technology has become a hot spot in recent years because of its strong sample transmission ability. Conversion screen is the key component of neutron radiography device. Optical fiber conversion screen is a new type of fast neutron radiography conversion screen, which takes into account the high detection efficiency of fiber array and the high imaging quality of the fluorescent screen, and has a good application prospect. Using D-T accelerator as neutron source, fast neutron radiography optical fiber conversion screen was developed by ZnS, epoxy resin and optical fiber, coupled with scientific CCD digital imaging system. The fast neutron radiography technology was studied. The integrated exposure images of optical fiber conversion screen with different optical fiber arrangement modes were obtained. Meanwhile, the detection efficiencies of fast neutron fluorescence screen and plastic scintillator were measured. The result shows that the detection efficiency of optical fiber conversion screen is better than that of other conversion screen.