Abstract: In this paper, the optimal pre-heat temperature of quartz about 375 ℃ thermoluminescence (TL) peaks at different irradiation doses and the optimal test dose of quartz aliquots about correcting thermal sensitivity were analyzed based on analysis of TL characteristics of quartz. According to the measurement results, the conventional protocol was improved. The improvement of the accuracy of equivalent dose measurement was verified based on the improved protocol of optimal measurement parameters. The experiment results show that if equivalent dose of quartz is small, the signal of 325 ℃ TL peak is weaker, and the lower pre-heat temperature can reduce the impact from 325 ℃ TL peak for 375 ℃ TL peak. If the equivalent dose of quartz is larger, in order to reduce the impact from 325 ℃ TL peak, the pre-heat temperature of regeneration dose and test dose should be set separately, and the measurement protocol should determine the optimal preheat temperature of quartz before the equivalent dose measurement. When the regeneration dose of quartz aliquots is in the range of 0-1 000 Gy, the test dose of 200 Gy can correct thermal sensitivity change of quartz aliquots and the relative deviation of 10 repeated measurements is less than 5%. The residual signal interference of 325 ℃ TL peak can be reduced and the quartz thermal sensitivity change can be corrected by using the above optimal pre-heat temperature and test dose. The relative deviations of the equivalent dose of 400 Gy and 700 Gy under the conventional protocol are 14.74%-47.15% and 33.47%-197.71%, and the relative deviations of the equivalent dose are all reduced to the range of ±4% under the improved protocol. The research provides an important reference for improving the measurement accuracy and the range of dating of 325 ℃ TL peak measurement.