Abstract: In this paper, a simultaneous and rapid analysis method of 89Sr and ⁹⁰Sr was established by proportional counter to measure twice after separation using Sr specific resin. The effects of ⁹⁰Y growth factor and ⁸⁹Sr/⁹⁰Sr activity ratio on the measured values of 89Sr and ⁹⁰Sr were studied. The results show that increasing ⁹⁰Y growth factor is more conducive to obtain the activities of ⁸⁹Sr and ⁹⁰Sr. This method was applied to the analysis of ⁸⁹Sr and ⁹⁰Sr in samples with ⁸⁹Sr/⁹⁰Sr activity ratio ranging from 0.22 to 21.8, and the relative deviation between the measured value and the standard value is less than ±30%. The uncertainty analysis shows that the uncertainty of one nuclide will increase with activity ratio or activity of another nuclide. The maximum relative deviation of ⁸⁹Sr and ⁹⁰Sr between the measured value and the standard value is 7.44%, which proves the accuracy of this method by the analysis of IAEA proficiency test samples.