Abstract: The effect of calculation conditions, such as grid division, number of energy groups, number of angles and expansion order of scattering cross section, on the shape of adjoint neutron fluence rate was studied using a discrete ordinate code. By solving the adjoint transport equation, the 3D spatial response function (SRF)of the detector in China Experimental Fast Reactor was calculated. The effects of position of control rod, fuel burnup, number of fuel subassemblies loaded on the SRF were analyzed. The results show that shape of adjoint neutron fluence rate has low sensitivity to the grid width, and under a coarse grid condition it can be used to calculate SRF. The influence of the control rod on SRF is related to the position of the control rod in the core, and the degree at which SRF is affected by the control rod is related to the relative positions of the fuel subassemblies and the control rod. The SRF is obviously affected by the newly loaded fuel subassemblies, but the sensitivity between the SRF and the position of newly fuel loaded subassemblies is low. The results provide a reference for the calculation of SRF, and technical support for dynamic rod worth measurement in large fast reactors.