Abstract: Disinfection or sterilization is an important part of the production process of medical disposable protective clothing (referred to as medical protective clothing). Compared with ethylene oxide sterilization method, irradiation is faster and more efficient. Since medical protective clothing has strict requirements in terms of protection performance, the radiation effect of its materials has important research significance. The electron beam accelerator was used to treat three kinds of medical protective clothing materials polypropylene (PP), polyester fiber (PET) and Tyvek with different absorbed doses. Then the samples were subjected to aging tests for different time. The main performance indicators related to radiation effect in GB 19082-2009 were tested. Test results show that the performances of the three kinds of medical protective clothing such as resistance to water penetration, resistance to synthetic blood penetration, and filtration efficiency are not significantly affected when absorbed dose is below 50 kGy. However, the breaking strengths of PP and Tyvek materials protective clothings are greatly affected. Two-factor analysis of variance was used to study the significance of the effects of radiation and aging on the breaking strengths of the three kinds of medical protective clothing materials. Results show that the absorbed dose and the aging time after irradiation have different effects on the breaking strength of the three protective clothing materials. Among them, the absorbed dose has the most significant radiation effect on the breaking strength of PP protective clothing. It proves that improving the initial breaking strength of PP protective clothing can make its performance meet the standard after irradiation.