Abstract: The test of the biological distribution of radiopharmaceutical technetium ⁹⁹Tc^mmethylene diphosphonate injections plays crucial roles in the determination of drug efficacy. In this work, a study of biological distributions regarding the technetium ⁹⁹Tc^mmethylenediphosphonate injections labeled by different domestic manufacturers was performed to establish a test method suitable for the test of domestic technetium ⁹⁹Tc^mmethylenediphosphonate injections. The test conditions of dissection time and injection dose were screened. The results indicate that the uptake ratios of these technetium ⁹⁹Tc^mmethylenediphosphonate injections at different organs are nearly consistent when the injection doses are ranging from 1.48 MBq to 74 MBq, and would reach equilibrium after two hours. The test method of technetium ⁹⁹Tc^mmethylenediphosphonate injections was established through the above research. The results show that the test method is accurate and reliable, and can be used as one of the quality control indexes of technetium ⁹⁹Tc^mmethylenebisphosphonate injection, and can be used for its quality control.