Abstract: Metallogenic chronology is one of the most important contents in the study of uranium deposits, it is a critical point to determine the age of uranium deposits. In this study, with the help of secondary ion mass spectrometer (SIMS) platform, the U-Pb in-situ technology for uraninite was estabished. In order to solve the complex genesis of uranium minerals, the spatial resolution of the pimary beam spot about 10 μm×5 μm was obtained. The reasonable of using GBW04420 was discussed, and it is proved that the GBW04420 has some limitations when used as the standard for SIMS dating. Using the zircon 91500 as the reference to find the secondary ion peak intensity and location were determined, and the target prepartion process for sample target for SIMS dating was proposed. The ²⁰⁷Pb-²⁰⁶Pb age of uranium deposit in China were obtained under the condition of high spatial resolution. Compared with other test results, it is proved that the construction of SIMS U-Pb uranium minerals method is reliable and reasonable.