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ZHANG Xue-feng, LIU Yi-bing, JIA Juan-juan, XU Wen-ge, LI Zi-ying, CHEN Yong-li, HAN Shi-quan. Chemiluminescence Immunoassay for Prostate-Specific Antigen[J]. Atomic Energy Science and Technology, 2008, 42(2): 112-116. DOI: 10.7538/yzk.2008.42.02.0112
Citation: ZHANG Xue-feng, LIU Yi-bing, JIA Juan-juan, XU Wen-ge, LI Zi-ying, CHEN Yong-li, HAN Shi-quan. Chemiluminescence Immunoassay for Prostate-Specific Antigen[J]. Atomic Energy Science and Technology, 2008, 42(2): 112-116. DOI: 10.7538/yzk.2008.42.02.0112
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Chemiluminescence Immunoassay for Prostate-Specific Antigen

  • China Institute of Atomic Energy, P.O. Box 275-35, Beijing 102413, China

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  • Received Date: May 09, 2007
  • Revised Date: August 12, 2007
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    • Abstract
  • The chemiluminescence immunoassay (CLIA) for serum total prostate-specific antigen (T-PSA) was developed. The reaction of luminol with hydrogen peroxide was introduced into this chemiluminescence system. The detection limit is established as 0.12 μg/L (n=10, mean of zero standard + 2SD) and the analytical recovery of PSA is 83.8%-118.7%. The intra-assay and inter-assay CVs vary from 4.4%-5.0% and 6.2%-11.7%, respectively. The experimental correlation coefficient of dilution is found to be 0.999. Compared with immunoradiometric assay (IRMA) kits, the correlative equation is y=1.07x+0.68, and correlation coefficient r= 0.97. The standard range for the method is 1.5-80 μg/L, and it presents good linearity.
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    • References (10)
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